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Zuhause> Blog> The Feature of Sanger Sequecing Instrument

The Feature of Sanger Sequecing Instrument

August 23, 2023

Molecular Biolab Equipment Display

Dna Analyzer

The Sanger sequencing instrument possesses several key features that enable the accurate and efficient sequencing of DNA. Some of the notable features include:

1. Laser and fluorescent detection: The instrument is equipped with a laser that excites fluorescently labeled nucleotides during the sequencing reaction. The emitted fluorescent signals are detected by sensitive detectors, allowing for the identification of the terminating nucleotides.

2. Capillary electrophoresis: The Genetic Analyzer Forensic Dna Instrument utilizes capillary electrophoresis to separate DNA fragments based on their size. It employs a capillary tube with a polymer matrix and applies an electric field to facilitate the migration of DNA fragments. This separation process enables the determination of the order of nucleotides in the DNA sequence.

3. Automated sequencing process: The Sanger sequencing instrument automates various steps of the DNA sequencing process, reducing human error and increasing efficiency. It can perform DNA amplification, primer annealing, and extension, as well as the separation and analysis of DNA fragments.

4. High accuracy: Sanger sequencing is known for its high accuracy, and the sequencing instrument contributes to maintaining this accuracy. The instrument detects and analyzes the fluorescent signal emitted by each terminating nucleotide, allowing for precise determination of the order of nucleotides in the DNA sequence.

5. Electropherogram generation: The instrument generates an electropherogram, which is a graphical representation of the DNA sequence. The electropherogram displays peaks that correspond to the different nucleotides, allowing researchers to visualize and interpret the DNA sequence.

6. Compatibility with different DNA samples: The Sanger sequencing instrument can handle various types of DNA samples, including individual genes, plasmids, and sections of genome assemblies. This versatility enables researchers to sequence a wide range of DNA fragments for different applications.

Although the Sanger sequencing instrument has been largely replaced by next-generation sequencing technologies for high-throughput sequencing projects, it remains a valuable tool for smaller-scale sequencing tasks requiring high accuracy and reliability.

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